ASSAY ON WHITE BLOOD CELLS PARAMETERS OF STREPTOZOTOCIN-INDUCED FEMALE DIABET(C ALBINO WISTAR RATS TREATED WITH BIOSYNTHESIZED SILVER NANOPARTICLES
Abstract
Diabetes mellitus is a type of metabolic disorder characterized by abnormal carbohydrate
metabolism resulting in chronic hyperglycemia caused by defective insulin production.
Diabetes mellitus and its complications are a major cause of early disease and death worldwide.
Silver nanoparticles (AgNPs) have garnered considerable attention in recent years for their
diverse biomedical applications, including antimicrobial, anti-inflammatory, and anticancer
properties. This study investigates the effects of biosynthesized silver nanoparticles on white
blood cell parameters in streptozotocin-induced diabetic female albino Wistar rats. Thirty
female (30) albino Wistar rats weighing (120-230g) were obtained from the University animal
house and assigned to five (V) groups with 6 rats in each group after feeding and
acclimatization for two weeks with commercially purchased pelletized animal feed. Group IIV
were induced intraperitoneally with STZ at a dose of 60mg/kg after an overnight meal fast
and observed for 48hrs to induce diabetes before treatment. Blood plasma glucose was tested
with one touch glucometer using blood collected from tail puncfure. Random blood glucose of
200 mg/dL and above was confirmation for diabetes induction. Animal groups were labelled
as follows; Group I: Normal control (NC), Group [1: Diabetic control (DC), Group I1l: Standard
control (MET), Group IV: Silver nanoparticle (AgNPs) and Group V: Guava leaves extract
(GV). At the end of the 14-days treatment period, study animals were sacrificed after being
anesthetized with 50:50 chloroform and diethyl ether. Blood was collected through cardiac
puncture for white blood cell count, leucocytes and lymphocyte concentration. The results
analysis showed that AgNPs treatment group showed significant (p
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